Extended Data Fig. 3: Satb2−/− control for FISH and IHC and neocortex-specific Satb2 transcription.
From: A critical period of translational control during brain development at codon resolution
a, Fluorescence in situ hybridization (FISH) and immunohistochemistry (IHC) probing for Satb2 and Bcl11b mRNA and protein, respectively, in wild-type (Satb2+/+) and Satb2 knockout (Satb2−/−) neocortex coronal sections at E14.5. Ventricular zone (VZ), cortical plate (CP). b, Measurement of Satb2 and Bcl11b mRNA cluster sizes in FISH probed neocortex sections at three developmental stages. Intermediate zone (IZ), lower layers (LL), upper layers (UL). Center is median, bounds are quartiles. c, Satb2 transcription activation visualized in Satb2Cre/+ mice by in utero co-electroporation of the neocortex and ganglionic eminence with a loxP-STOP-loxP-tdTomato (Satb2tdTom) fluorescence reporter at E12.5, along with eGFP reporter for all transfected cells, and analysis in coronal sections at E13.5. Sub-ventricular zone (SVZ). Associated with Figs. 2 and 3. See also Supplementary Table 3.
