Fig. 2: Translation upregulation of Satb2 leads to divergent spatiotemporal mRNA and protein expression.
From: A critical period of translational control during brain development at codon resolution
a, GO (molecular function, MF) analysis of translationally upregulated (TE up) mRNAs. b, The median trajectory of Satb2, Nes and Bcl11b gene expression measured by RNA-seq, Ribo-seq, mass spectrometry and translation efficiency. The E15.5 time point is highlighted for Satb2. c, Satb2, Nes and Bcl11b expression in scRNA-seq data tracking differentiating neocortex cells from 1 h (apical progenitor, AP) to 96 h (neuron 4 days old, N4d) after birth (y axis), at birthdates E12, E13, E14 or E15 (x axis), with expression levels calculated in ref. 4. Expected distribution of protein expression2 is outlined. d, Neocortex coronal sections at E12.5, E14.5 and E16.5 analyzed for Satb2 and Bcl11b mRNA by FISH, and protein by IHC. Deep border of the cortical plate is demarcated at E16.5 (dotted line). Nuclei are stained with DAPI. CP, cortical plate; VZ, ventricular zone; UL, upper layers; LL, lower layers. e, Quantification of d; n = 3 independent brains for E16.5 IHC, n = 4 independent brains for all other stages or assays. Mean ± s.d. is shown. Comparison of adjacent cortical layers starting from deep (VZ) to superficial by unpaired two-tailed t-test with Welch’s correction, or Mann–Whitney U-test, after Shapiro–Wilk normality test. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. See also Extended Data Fig. 3a,b and Supplementary Table 3.
