Fig. 4: Translation downregulation decreases ribosome levels acutely at mid-neurogenesis.
From: A critical period of translational control during brain development at codon resolution
a, GO (molecular function) analysis of translationally downregulated (TE down) mRNAs. b, The expression trajectories (gray) of all 79 ribosomal protein coding mRNAs in the large (Rpl) and small (Rps) subunits from E12.5 (t0) to subsequent stages (t), measured by RNA-seq, Ribo-seq, mass spectrometry and calculated translation efficiency. Median trajectories are shown in black. c,d, Immuno-electron microscopy (immuno-EM) labeling ribosomal protein uS7 (magenta) in the E12.5 and E15.5 neocortex neural stem cells and neurons (c), with quantification for ribosomes per cytoplasmic area (d); n = electron microscopy images, captured from 2 independent brains, 2 sections per brain at each developmental stage. Mean is shown (line), two-way Welch’s ANOVA and Dunnett’s post hoc test, **P < 0.01, ***P < 0.001. Neural stem cells are located in the ventricular zone (VZ) and subventricular zone (SVZ); post-mitotic neurons are located in the cortical plate (CP), lower layers (LL) and upper layers (UL). Nuclei are outlined. See also Extended Data Fig. 4 and Supplementary Table 3.
