Fig. 5: Ribosome density at the start codon and in the coding sequence shifts sharply at mid-neurogenesis.
From: A critical period of translational control during brain development at codon resolution
a, Ribosome occupancy metagene plot including all mRNAs (top) surrounding the start (left) and stop (right) codons at five stages. Separation of mRNAs by changing or unchanged start codon occupancy (bottom). b, Position-specific fold changes in ribosome P-site counts surrounding the start and stop codons. c, Start (left) and stop (right) codon occupancy vs translation efficiency fold change per gene. Center is the maximum likelihood slope; ribbon is the 95% confidence interval. d, Between-codon variance in ribosome occupancy of A-sites, P-sites and E-sites at each stage. Calculation with both 29 nt (top) and 30 nt (bottom) RPF fragments shown. e, Distribution of per-codon A-site and P-site occupancy at each stage. f, Correlation between A-site and P-site occupancy per codon. Center is the maximum likelihood slope; ribbon is the 95% confidence interval. g, Ribosome A-site occupancy for each amino acid with corresponding synonymous codons at each stage. See also Extended Data Figs. 5 and 6 and Supplementary Table 4.
