Extended Data Fig. 2.: Cryo-EM structural determination of the SAMTom40-early intermediate.
From: A multipoint guidance mechanism for β-barrel folding on the SAM complex
a, Representative cryo-EM micrograph image of the SAMTom40-early intermediate. The image is a representative of over thousand images. b, Schematic workflows of the steps followed in data processing leading to the final structures of the SAMTom40-early intermediate. c, Cryo-EM maps of the SAMTom40-early intermediate for different classes (b) filtered by different local resolutions (6σ and 9σ). Local resolution filtered maps at a lower threshold level (1σ) are overlaid in grey. Particle numbers and overall map resolutions are shown on the left. d, Upper left, local map resolutions of the final structures (class 3), with colors according to the scales. Upper right, FSC curves between the EM density maps and the atomic models of the SAMTom40-early intermediate (class 3). Lower, FSC curves between the EM density maps and the atomic models for the class 2 and 5 structures.
