Extended Data Fig. 5: Labeling of httex1 with fluorinated glutamines.
From: The structure of pathogenic huntingtin exon 1 defines the bases of its aggregation propensity
(a) Enzymatic loading of suppressor tRNACUA with canonical (Gln) or 2 S,4R-fluoroglutamine (4F-Gln). Upper and lower bands correspond to loaded and unloaded suppressor tRNACUA, respectively. A negative control of empty tRNACUA is shown in the third lane. (b) Averaged fluorescence endpoint derived from n = 2 independent cell-free suppression reactions of H16 with a stop codon at position Q18 or Q21 when titrating with increasing concentrations of both Gln-tRNACUA and 4F-Gln-tRNACUA. “+” indicates a positive control, a cell-free reaction of H16 without any amber stop codon. Data are presented as mean values + /- standard deviation.
