Extended Data Fig. 4: Conformational change is triggered by structured RNA substrates with shortened 3′ overhangs.
a, Predicted RNA-fold for hairpinD-U7 (75%- GC content of dsRNA) and hairpinE-U7 (50% GC content of dsRNA). b, Final 3D map of human Dis3L2 in complex with hairpinD-U7 superimposed with the structure of RNA-free Dis3L2 fit in the map. c, GSFSC and d, particle viewing direction distribution. The final 3D map was produced by homogeneous refinement of roughly 539,000 particles in cryoSPARC. e, Urea-PAGE of nuclease activity assay using pre-let7-U15, hairpinD-U7 and hairpinE-U7 as substrates. Species length is indicated on the left of the gel. f, Unsharpened map of Dis3L2 in complex with the 50% GC-rich hairpinE-U7 (homogeneous refinement with 226,117 particles) with structure of Dis3L2-hairpinD-U7 fit in the map. g, Density overlay of the S1 domain. h, Density for the CSDs in the vase conformation i, and in the prong.
