Extended Data Fig. 9: Comparison of structures and kinetic profiles of human Dis3 and Dis3L2.
a, Structures of Human Dis3L2 and Human Dis3 (PDB code: 6D6Q)51. b–f, Kinetic profile for hairpinA-GCU14 degradation by HsDis3 at single-nt resolution: b, Processivity (blue circles), c, Functional equilibrium dissociation constant for productive binding (K1/2) (green diamonds), d, forward rate constant (orange squares), e, dissociation rate constant (pink triangles), and, f, association rate constants (purple circles) compared to HsDis3L2 (gray). Error bars for (d-f) represent the SEM derived from a global fit of data from enzyme titrations (9 Dis3 concentrations with n = 2) and pulse-chase experiments (2 Dis3 concentrations with n = 3). Processivity error bars for (b) are propagated errors calculated from the SEM of kf and koff, while K1/2 errors (c) are propagated errors calculated from SEM of koff and kon.
