Fig. 2: Structured RNA is not engaged with Dis3L2 upon initial binding to the 3′ oligo-U tail. | Nature Structural & Molecular Biology

Fig. 2: Structured RNA is not engaged with Dis3L2 upon initial binding to the 3′ oligo-U tail.

From: A shape-shifting nuclease unravels structured RNA

Fig. 2

a, RNA fold of hairpinA–GCU14. b, Cryo-EM structure of Dis3L2D391N in complex with hairpinA–GCU14. c, Alignment showing the active site of MmDis3L2 in complex with U13 (gray) (PDB: 4PMW) and HsDis3L2 in complex with hairpinA–GCU14. d, The hydrogen bond network from C20–U25 traverses the apical face of the protein and involves both CSDs and the S1 domains. e, Following U25, the RNA enters into the narrow portion of the channel. f, The hydrogen bond network continues within the RNB all the way to the active site. For C20–U32, these include base-specific hydrogen bond interactions.

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