Extended Data Fig. 7: RPA condensates concentrate RAD52 in vitro. | Nature Structural & Molecular Biology

Extended Data Fig. 7: RPA condensates concentrate RAD52 in vitro.

From: Phase separation properties of RPA combine high-affinity ssDNA binding with dynamic condensate functions at telomeres

Extended Data Fig. 7

a, Purified RAD52 partitions into RPA droplets in vitro. Purified trimeric unlabeled RPA was allowed to form liquid droplets prior to incubation with Cy3-labeled purified RAD52. Time-resolved partitioning of RAD52 into RPA droplets was followed by time-lapse microscopy. Cy3-labeled RPA served as positive control, confirming homotypic RPA interactions in phase separated RPA droplets. The Cy3 label added to preformed RPA droplets served as negative control. Cy3-labeled RPAΔN, lacking the N-IDR of RPA2, served as additional negative control and confirmed the involvement of the N-IDR in homotypic RPA interactions. Representative stills from time-lapse microscopy are shown. b, Purified RAD52 alone is not forming phase separated droplets in the conditions used in (a). c, Coomassie staining of the purified human RAD52 after SDS-PAGE. Scale bars 10 µm.

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