Extended Data Fig. 8: Localization of RPA and RAD52 at telomeres.
a, Co-localization of RPA2 and RAD52 at TRF2-marked telomeres in U-2 OS cells in unchallenged conditions and after treatment with 0.2 µM aphidicolin for 24 h. b, Enhanced co-localization of RPA2 and RAD52 at TRF2-marked telomeres in U-2 OS cells upon depletion of FANCM. c, Quantification of RPA at telomeres. Co-localization of RPA with TRF2-marked telomeres in U-2 OS cells in unchallenged conditions and after treatment with 0.2 µM aphidicolin for 24 h. Two-tailed unpaired t-test, ns p = 0.7898. d, As in (c) in U-2 OS cells transfected with negative control siRNA or siRNA against FANCM. Two-tailed unpaired t-test, ** p = 0.0049. e, Percentage of TRF2 foci co-localizing with RPA in cells analyzed in (c). Two-tailed unpaired t-test, ** p = 0.0028. f, Percentage of TRF2 foci co-localizing with RPA in cells analyzed in (d). Two-tailed unpaired t-test, **** p < 0.0001. g, Percentage of RAD52 foci co-localizing with TRF2-marked telomeres in cells treated as in (c). Two-tailed unpaired t-test, ns p = 0.1824. h, Percentage of RAD52 foci co-localizing with TRF2-marked telomeres in cells treated as in (d). Two-tailed unpaired t-test, *** p = 0.0002. i, Percentage of TRF2 foci co-localizing with RAD52 in cells analyzed in (g). Two-tailed unpaired t-test, **** p < 0.0001. j, Percentage of TRF2 foci co-localizing with RAD52 in cells analyzed in (h). Two-tailed unpaired t-test, **** p < 0.0001. c-f, Averages and standard deviation from n = 3 independent samples with 50 analyzed cells per condition and replicate are shown. g-j, Averages and standard deviation from n = 3 independent samples with (g,i) WTcontrol n1 = 92, n2 = 96, n3 = 107; WTAPH n1 = 80, n2 = 73, n3 = 101; S/T→Dcontrol n1 = 149, n2 = 108, n3 = 90; S/T→DAPH n1 = 88, n2 = 81, n3 = 86 (h,j) WTsiCON n1 = 134, n2 = 145, n3 = 1115; WTsiFANCM n1 = 80, n2 = 68, n3 = 72; S/T→DsiControl n1 = 117, n2 = 108, n3 = 114; S/T→DsiFANCM n1 = 78, n2 = 83, n3 = 65 cells are shown.
