Extended Data Fig. 5: Molecular combing analysis of the FHIT locus, principle and examples. | Nature Structural & Molecular Biology

Extended Data Fig. 5: Molecular combing analysis of the FHIT locus, principle and examples.

From: Mistimed origin licensing and activation stabilize common fragile sites under tight DNA-replication checkpoint activation

Extended Data Fig. 5

A: Map of the FHIT gene, with the position its 10 exons (ex) and of the 29 short probes (l5.5 to 6.6 Kb)-long - dark green rectangles), organized in five motifs (a-e) that constitute the FISH code bar initially used for identification of a region 1.6 Mb-long encompassing the gene52. Six longer probes (from left to right, size in kb L1: 40, L2: 50, L3: 40, L4: 40, L5: 60 and L6: 30) were recently added to facilitate screening (light green rectangles). B: Examples of DNA fibres bearing FISH tracks (revealed in green) and replication signals (pulse 1: IdU in blue, pulse 2: CldU in red) from cells treated as Fig. 1e. Three colour-painting and FISH alone are shown. A schematic representation of the fiber (in grey) is also presented, arrowheads indicate the direction of fork progression and blue squares the estimated positions of initiation events.

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