Extended Data Fig. 6: Supplementary results relative to western blots.
A-D: MCM7 protein accumulates on chromatin of S6/G2 cells grown in ARO. A: Experimental scheme. B: Quality-control of the fractionation procedure. Ponceau staining is shown as a loading control and size of molecular weight markers is indicated. The levels of GAPDH (cytoplasmic marker) and histone H4 (nuclear marker) were analysed with corresponding antibodies. Experiment was done once. C: Cells were treated as in A, fractionated and protein extracts were analysed with MCM7 antibodies. Two biologically independent experiments were done with similar results. D: Biologically independent duplicate of the experiment shown in Fig. 4g (middle panel, chromatin). E: Kinetics of CDC6 and CDT1 depletion. JEFF cells were transfected with siCDC6, siCDT1 or sictrl. At indicated times after transfection, total protein extracts were prepared to assess the level of each factor. Beta-actin was used as a loading control. Experiments were done once.
