Extended Data Fig. 9: Examples of metaphase plates observed in experiments of Fig. 5b.
A: Upper panel: Experimental scheme. Lower panels: Protein extracts from cells used in Fig. 5b (two biologically independent experiments) were prepared to assess the level of each factor. Ponceau staining was used as a loading control. B: Examples of BrdU-labelled and unlabelled metaphases plates observed in the two biologically independent experiments shown in Fig. 5b. Note that the labelling reveals classical replication bands, showing that cells were engaged in the S-phase during the pulse. Scale bars: 20 μm.
