Extended Data Fig. 3: Analysis of Repli-Seq data.
A: Representative FACS profile showing the gate position for cell sorting. Fluorescent units are reported on the x axis and the cell counts on the y axis. The percentage of replicated DNA (based on the G1 and G2 peaks) is shown at the top. B: Simulation of S-phase progression (based on72). Blue curve: percentage of replicated DNA shown as a function of simulation cycle. Red curve: typical bell-shaped curve given by the ratio between origin firing and un-replicated DNA as a function of simulation cycle. C: Density of active forks in function of the percentage of replicated DNA obtained in the simulation. The relative quantity of newly replicated DNA inside each fraction has then been used to normalize the Repli-Seq data. D: Heat-map showing Pearson correlation coefficient between individual biological replicate of NT, Aph and ARO samples. E: Repli-Seq profiles of FRA3B and FRA16D showing the profiles of individual replicates in each condition in grey, and the average profiles in blue for NT, green for Aph and orange for ARO (as in Fig. 1b, c). Biologically independent experiments were performed three times for NT, twice for Aph and ARO with similar results. Data are presented as mean values.
