Extended Data Fig. 2: Three-dimensional reconstruction pipeline and quality of cryo-EM maps. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Three-dimensional reconstruction pipeline and quality of cryo-EM maps.

From: Mechanism of RecF–RecO–RecR cooperation in bacterial homologous recombination

Extended Data Fig. 2

(a) RecF-DNA, (b) RecF-RecR-DNA, (c) RecO-RecR-DNA, and (d) RecF-RecO-RecR-DNA. (e) Top: gold-standard Fourier Shell Correlation (FSC) curves between two half maps, model-to-map FSC curves, and histograms of directional FSC (calculated by the 3DFSC web-server64). Horizontal lines represent a value of 0.143. Bottom: viewing direction distribution graphs. (f–i) Local resolution calculated from half maps in cryoSPARC for RecF-DNA (f), RecF-RecR-DNA (g), RecO-RecR-DNA (h) and RecF-RecO-RecR-DNA (i) reconstructions.

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