Extended Data Fig. 3: Overall structure of RecF-DNA complex and comparison with the crystal structure.
From: Mechanism of RecF–RecO–RecR cooperation in bacterial homologous recombination
(a) High-resolution cryo-EM potential map of RecF-DNA complex. The two RecF protomers are displayed as orange and dark pink, and DNA is in white. (b) Two views of the RecF-DNA complex shown in cartoon representation. The color scheme is the same as in (a). The DNA is in black. (c) Side view of superposition of RecF structures. RecF-RecR-DNA reconstruction (present study) is shown in yellow/sand for RecF and purple/cyan/pink for RecR. The DNA is in black. RecF structure from RecF-DNA reconstruction (present study) is shown in same color scheme as in (a). The apo RecF structure (PDB ID: 5Z68) is shown in green. The proteins were superimposed using the ATPase domains and are shown in wire representation. The helical clamp is shown in cartoon representation. (d) Bottom view of superposition of RecF structures to show the difference in clamp placement. The color scheme is the same as in (c).
