Extended Data Fig. 4: SNX1BAR-SNX5BAR interface validation.
(a) SEC-MALS analysis of F347A, W511A double mutant on the SNX1 (SNX1†) interface that precludes its homodimerization. (b) Affinity pull down assay for mutants influencing SNX1 heterodimerization. (c) Tandem affinity purification of SNX5 induced homodimers. SNX5WT was tagged with His-SUMO, and single (*) or triple (**) SNX5 mutants were tagged with GST. The complex was purified from combined cell lysates. Left lane shows the dimer with the His-SUMO tag still on SNX5. (d) Liposome tubulation ability of SNX1 and SNX5 proteins with mutations at interfaces affecting their dimerization capacity. Results in a-d are representative of two independent experiments.
