Extended Data Fig. 8: Height of intersegment and mismatch barriers in the energy landscape.
From: The energy landscape for R-loop formation by the CRISPR–Cas Cascade complex
a, (Top) Free energy barriers for intersegment transitions for the T6, T6-flipG and T6-flipT targets (see legend). (Bottom) The height of the transition barriers of a particular free energy landscape was determined from its difference to a barrier-free reference obtained by connecting the minima of adjacent free energy wells (dashed lines). The resulting barriers were approximately centred on the flipped base positions (dotted grey lines). Shaded areas correspond to s.e.m. The landscapes are shifted along the energy axis for better comparison. b, Free energy penalty introduced by a single C:A mismatch at position 17 between the target strand and crRNA. Shown are the apparent energy landscapes of the T6 (black) and the T6-M17 (green) targets as well as the difference between both apparent (purple) and both deconvolved (orange) energy landscapes. The difference between the two landscapes reveals a pronounced mismatch penalty, that is an offset of the energy values for lengths larger 17 bp, of 4.7 ± 0.4 kBT (see difference between the blue dashed lines). The landscapes are shifted along the energy axis for better comparison.
