Extended Data Fig. 1: Scheme and TEM images of the nanorotor.
From: The energy landscape for R-loop formation by the CRISPR–Cas Cascade complex
a, Three-dimensional scheme of the nanorotor including the origami rotor arm and a 50 nm AuNP. Blue cylinders represent the individual dsDNA helices. Insets at the top and bottom show enlarged perspectives as well as schematic views of the dsDNA ligation interfaces at each end of the stem, where two complementary ssDNA staple overhangs of neighbouring helices (orange) and a secondary interface oligo form a sticky end for ligation. Other helix ends carry six nucleotide ssDNA staple overhangs (purple) to prevent aggregation between rotor arm structures due to blunt end DNA stacking. The insets in the middle show detailed and schematic views of the attachment of the AuNP to the end of the rotor arm, in which the connecting DNA strands hybridize in a zipper-like configuration. b, TEM overview image of several DNA origami rotor arms and selected images of individual structures (top). c, TEM overview image of DNA origami rotor arms with attached 50 nm AuNPs and selected images of individual structures (top). The nanorotor binds often to protrusions on the AuNP. All TEM images are scaled to the same magnification. The length of the scale bars is 100 nm with 20 nm for the length of each black and white segment. TEM imaging was performed on 3 independent preparations.
