Extended Data Fig. 4: The scc2-pip mutant shows additive cohesion defects with ctf19Δ, the mutant of kinetochore receptor for cohesin loader, and is synthetic lethal with scc4Δ.
From: PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion
a-b, Tetrad dissection analysis of the indicated diploid strains. The scc2-pip mutant shows synthetic growth defect when combined with ctf18Δ ctf19Δ (b) in comparison with ctf18Δ ctf19Δ double mutant (a). c, Similar to ctf18Δ, the scc2-pip mutant shows additive sensitivity to benomyl when combined with ctf19Δ. d, Tetrad dissection analysis of the SCC2/scc2-E822K-17aa SCC4/pGALS-SCC4 diploid strain on galactose- (YP Gal) and glucose-containing (YPD) plates. scc2-E822K-17aa mutant lacking the last 17 residues that harbor consensus PIP motif cannot rescue the lethality of cells expressing SCC4 from the galactose-inducible pGALS promoter when tetrads are dissected on YPD plates. e, The disassembly-prone PCNA mutant pol30-D150E shows additive sensitivity to benomyl when combined with scc4 scc2-E822K double mutant.
