Extended Data Fig. 6: The scc4Δ scc2-E822K double mutant shows additive defects with PCNA mutants affecting its front face. | Nature Structural & Molecular Biology

Extended Data Fig. 6: The scc4Δ scc2-E822K double mutant shows additive defects with PCNA mutants affecting its front face.

From: PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion

Extended Data Fig. 6

a, C-terminally 3MYC-tagged PCNA (POL30-3MYC) variant shows additive sensitivity to benomyl when combined with scc4Δ scc2-E822K double mutant. b, The alphaFold-Multimer prediction of the interaction between Scc2 PIP and C-terminally 3MYC-tagged yeast PCNA (Pol30-3MYC). 3MYC interferes with the interaction by affecting the front face of the PCNA ring. c-d, Tetrad dissection analysis of the indicated diploid strains. Mutants causing disruptions of a surface cavity on the front face of the PCNA ring, pol30-6 (c) and pol30-79 (d), show synthetic sickness when combined with scc4Δ scc2-E822K double mutant. e, pol30-79 mutant shows additive sensitivity to benomyl when combined with scc4Δ scc2-E822K double mutant.

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