Extended Data Fig. 9: The alphaFold-Multimer predictions of the interaction between PCNA and the C-terminal PIPs of cohesin loaders conserved from yeast to vertebrates.
From: PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion
The alphaFold2 prediction of the budding yeast cohesin loader Scc2 next to PCNA ring with its front face positioned towards the flexible C-terminal PIP motif of Scc2 colored red (upper left panel). The interactions of the C-terminal PIPs of the budding yeast S. cerevisiae cohesin loader Scc2 (residues 1476-1493), the fission yeast S. pombe cohesin loader Mis4 (residues 1553-1587), and the chicken G. gallus cohesin loader NIPBL (PIP3 residues 2621-2640; PIP2 residues 2764-2783) with PCNA predicted using alphaFold-Multimer (bottom panels from left to right; C-terminal protein ends are labeled with C). The PIPs are located within flexible C-terminal tails of cohesin loaders, the cryo-EM structures of which were not resolved previously (PDB 6ZZ6 – S. cerevisiae; PDB 6YUF – S. pombe; PDB 6WGE – H. sapiens) due to their mobility.
