Fig. 6: PCNA-guided recruitment of the chicken cohesin loader NIPBL to chromatin is required for SCC.
From: PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion
a, Sensitivity assay using CellTiter-Glo. The nipbl-ΔC-EGFP mutant exhibits hypersensitivity to nocodazole treatment. The mean values of three independent experiments ±s.d. are plotted. b, Frequency of chromosome loss was measured by the minichromosome-loss assay. Minichromosome 2 (miniChr.2) carries an mCherry expression unit, and the percentage of mCherry-negative cells was determined by flow cytometry. The mean values of three independent experiments ±s.d. are plotted. c, Growth curves showing synthetic lethality of nipbl-ΔC-EGFP with ddx11-aid but not with ctf18. The mean values of three independent experiments ±s.d. are plotted. Aux, auxin. d, Cohesion analysis of metaphase spreads. Depletion of DDX11 in the nipbl-ΔC-EGFP background results in severe cohesion defects. The mean values of three independent experiments ±s.d. are plotted.
