Extended Data Fig. 1: Elevated PCNA levels in elg1Δ do not promote SCC through Srs2 recruitment, but bring another factor to support viability of pds5Δ cells.
From: PCNA recruits cohesin loader Scc2 to ensure sister chromatid cohesion
a, Overexpression of SCC1 from galactose-inducible pGAL1 promoter (Gal 0.1%) supports viability of elg1Δ pds5Δ cells, whereas low SCC1 expression (Gal 0.01%) that supports viability of elg1Δ cells does not permit the growth of the elg1Δ pds5Δ mutant. Spotting of 1:7 serial dilutions on indicate plates containing glucose (YPD), raffinose (Raf) and galactose (Gal). b-c, Tetrad dissection analysis of the indicated diploid strains. Loss of Srs2 in elg1Δ wpl1Δ pds5Δ cells results in lethality that can be rescued by Rad51 deletion (b), whereas expression of the srs2-ΔC138 mutant unable to interact with PCNA does not cause lethality in elg1Δ wpl1Δ pds5Δ cells (c). d, Control of the expression of N-terminally 3HA-tagged Rad51 recombinase from the galactose-inducible promoter pGAL1 in the indicated backgrounds after 24 h shift from glucose-containing YPD to 2% galactose-containing YP Gal media.
