Extended Data Fig. 4: The iDDR prevents Apollo-independent nucleolytic processing of leading-end telomeres independently from 53BP1.
From: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres
(a) Telomeric overhang assay and quantification from one representative experiment in SV40LT-immortalized Trf2F/F 53BP1-/- RsCre-ERT1 MEFs expressing the indicated MYC-Trf2 alleles at 96 h after 4-OH tamoxifen (4-OHT)-mediated deletion of endogenous TRF2. For each allele, the normalized no Cre value was set to 1, and the + Cre value was given relative to it. (b) and (c) Telomeric overhang assay and quantification of ApolloF/F and ApolloF/F 53BP1−/− MEFs 96 h after Hit & Run Cre-mediated deletion of Apollo as described in (a) and (b), with means and SDs across three independent experiments. Statistical analysis by two-way ANOVA.
