Extended Data Fig. 6: The iDDR of TRF2 and the MIN domains of Rif2 and Taz1 are an example of convergent evolution.
From: DNA-PK and the TRF2 iDDR inhibit MRN-initiated resection at leading-end telomeres
(a) Schematic for telomere binding proteins in H. sapiens, S. pombe, and S. cerevisiae with TRF2, Taz1, Rif2 highlighted in purple. (b) AlphaFold-Multimer Predicted Aligned Error (PAE; top; representative of five ranked models generated with default parameters) plot for S. cerevisiae Rif2 and Rad50 and predicted Local Distance Difference Test (pLDDT; bottom) plot for each residue in Rif2 from the ranked models.(c) AlphaFold-Multimer PAE (top; representative of five ranked models generated with default parameters) plot for S. pombe Taz1 and Rad50 and pLDDT (bottom) plot for across Taz1 from the ranked models. (d) Representative AlphaFold-Multimer models of TRF2-Rad50 (left), Rif2-Rad50 (middle), and Taz1-Rad50 (right). Acidic (red) and basic (blue) residues are highlighted. (e) MUSCLE alignment of the iDDR domains from representative metazoans, highlighting the presence of basic residues (blue) followed by acidic residues (red). (f) Phylogenetic tree of Opisthokonts showing the emergence of the iDDR of TRF proteins (blue) as well as the MIN of Taz1 (green) and the MIN (or BAT) of Rif2/Orc4 (red). Whether other Opisthokonts independently evolved an iDDR-like motif is unknown. Not to scale.
