Extended Data Fig. 1: Cryo-EM data processing workflow for GluA2–γ5 and GluA2–γ5–CNIH2 in the presence of the competitive antagonist ZK200775.

A representative micrograph for the protein purified from HEK293S GnTI– cells transduced with the GluA2–γ5 virus, supplemented with 0.1 mM ZK and subjected to single-particle cryo-EM shows example particles circled in white.