Table 2 Cryo-EM data collection, refinement and validation statistics

From: Modulation of GluA2–γ5 synaptic complex desensitization, polyamine block and antiepileptic perampanel inhibition by auxiliary subunit cornichon-2

	GluA2–γ5–CNIH2_ZK-PMP-SPD LBD–TMD (EMDB-40746) (PDB 8SS7)	GluA2–γ5_ZK-PMP FL (EMDB-40747) (PDB 8SS8)	GluA2–γ5_ZK-PMP LBD–TMD (EMDB-40748) (PDB 8SS9)	GluA2–γ5–CNIH2_Glu-SPD FL (EMDB-40749) (PDB 8SSA)	GluA2–γ5–CNIH2_Glu-SPD LBD–TMD (EMDB-40750) (PDB 8SSB)
Data collection and processing
Magnification	×75,000	×75,000	×75,000	×105,000	×105,000
Voltage (kV)	300	300	300	300	300
Electron exposure (e⁻/Å²)	50	50	50	58	58
Defocus range (μm)	−1 to −2	−1 to −2	−1 to −2	−1 to −2	−1 to −2
Pixel size (Å)	0.925	0.925	0.925	0.83	0.83
Symmetry imposed	C2	C2	C2	C2	C2
Initial particle images (no.)	2,360,956	2,360,956	2,360,956	2,282,408	2,282,408
Final particle images (no.)	126,263	126,964	117,939	58,186	48,434
Map resolution (Å)	2.76	2.81	2.72	3.88	3.66
FSC threshold	0.143	0.143	0.143	0.143	0.143
Map resolution range (Å)	2.6/3.3/34.4	2.0/3.3/36.3	2.0/3.3/36.0	2.1/5.8/42.5	2.1/4.8/36.5
Refinement
Initial model used (PDB code)	7RZ5, 7OCE	7RZ5, 7OCE	7RZ5, 7OCE	7RZ5, 7OCE	7RZ5, 7OCE
Model resolution (Å)	2.76	2.81	2.72	3.88	3.66
FSC threshold
Map sharpening B factor (Å²)	−132.7	−135.5	−131.4	−101.4	−97.2
Model composition
Nonhydrogen atoms	19,436	28,487	16,319	30,888	19,048
Protein residues	2,266	3,514	1,986	3,840	2,304
Ligands
PMP	4	4	4	–	–
SPD	1	–	–	1	1
ZK	4	4	4	–	–
Glutamate (Glu)	–	–	–	4	4
B factors (Å²)
Protein	63.09	68.50	65.55	128.43	68.50
Ligand	41.07	98.06	65.93	66.30	98.06
R.m.s. deviations
Bond lengths (Å)	0.010	0.014	0.008	0.010	0.007
Bond angles (°)	1.657	1.585	1.541	1.431	1.376
Validation
MolProbity score	1.92	1.75	1.51	1.96	2.01
Clashscore	6.95	3.86	4.43	6.33	7.27
Poor rotamers (%)	1.45	1.33	0.36	1.00	0.72
Ramachandran plot
Favored (%)	93.80	92.19	95.80	87.63	87.37
Allowed (%)	5.66	7.12	3.89	11.37	11.40
Disallowed (%)	0.54	0.69	0.31	1.00	1.24

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