Extended Data Fig. 5: CRISPRi effects on APA.
a, CRISPRi targeted upstream of the terminal poly(A) site and gene expression level. RNA samples from Fig. 6a were reanalysed to estimate respective mRNA expression levels relative to control MYC gene expression and to upstream or downstream probe signal in the CTRL sample. Data from n = 2 biologically independent replicates are shown with mean value indicated on top of the bar. b, Screenshots from UCSC browser show representative Chr and PolyA+ RNA-seq profiles of HeLa cells transfected by CRISPRi CTRL or APA. CCND1 and PCMT1. c, Zoom-in of PCMT1 alternative PAS region. Dotted vertical lines show splice sites positions. Sashimi plot as in Extended Data Fig. 4d. d, Volcano plot of PolyA+ RNA-seq DE analysis (n = 3 biologically independent polyA+ libraries).
