Extended Data Fig. 5: Purification of structure-guided mutants of Cas1-2/3. | Nature Structural & Molecular Biology

Extended Data Fig. 5: Purification of structure-guided mutants of Cas1-2/3.

From: Structure reveals why genome folding is necessary for site-specific integration of foreign DNA into CRISPR arrays

Extended Data Fig. 5

a, SEC profile of a new preparation of wildtype Cas1-2/3 and all variants purified in the same manner on a Superdex 200 16/600 (Cytiva). An excess of free Strep-tagged Cas1 elutes at approximately 82 mL. b, SDS-PAGE gel of the Cas1-2/3 hetero-hexamer peak for all purified Cas1-2/3 variants. The SDS-PAGE gel of all Cas1-2/3 samples was run twice with similar results.

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