Extended Data Fig. 6: Sequence alignments of ELOA, ELOC and ELOB.

a. Alignment of ELOA proteins from different species, including human (Homo sapiens Hs ELOA1 and ELOA2, UniProt AC: Q14241, Q8IYF1), mouse (Mus musculus Mm ELOA1, UniProt AC: Q8CB77), chicken (Gallus gallus Gg ELOA, UniProt AC: A0A1D5PAE7), frog (Xenopus laevis Xl ELOA, UniProt AC: A0A1L8HFS2), zebra fish (Danio rerio Dr ELOA, UniProt AC: Q803L4), fruit fly (Drosophila melanogaster Dm ELOA, UniProt AC: Q9VCP0) and worm (Caenorhabditis elegans Ce ELOA, UniProt AC: Q09413). Yellow and grey stars indicate ELOA residues locating on the ELOA-ELOC interface and ELOA-Pol II interface, respectively. Residues with > 60% identity among species are highlighted with light and deep purple background. b. Alignment of ELOC proteins from different species, including human (Hs), mouse (Mm), chicken (Gg), frog (Xl), fruit fly (Dm), fish (Dr) and worm (Ce) with Uniprot AC or GenBank ID of Q15369, P83940, NP_001007889.1, XP_018098053.2, Q7JWD6, NP_001002440.2 and Q9BKS1, respectively. The species in ELOB and ELOC alignments are less than the ones in ELOA alignments, because ELOB and ELOC of some species are not present in the database. Residues with > 75% identity are highlighted with yellow background. Purple stars indicate ELOC residues locating on the ELOA-ELOC interface. c. Alignment of ELOB proteins from different species, including human (Hs), mouse (Mm), chicken (Gg), frog (Xl), fruit fly (Dm), fish (Dr) and worm (Ce) with UniProt AC or GenBank ID of Q15370, P62869, XP_040512765.1, NP_001080414.1, O44226, NP_001136426.1 and G5ECR7, respectively. Residues with > 75% identity are highlighted with salmon background. Secondary structure assignment is shown according to the structure of human ELOA, ELOB and ELOC from this study. Secondary structures were assigned by PyMOL dssp. Note: ELOB β3 and β4 were shown to be beta strands in other studies but not assigned by Pymol dssp in this study.