Extended Data Fig. 4: AGO2HA associates with chromatin in an RNA-dependent manner.
From: AGO2 silences mobile transposons in the nucleus of quiescent cells
(a) Western blot showing undetectable expression of AGO1 and AGO3 in primary mouse B cells. Lysates from two biological independent mouse embryonic fibroblast lines (MEF1 and MEF2) were used as control. Res., resting cells; Act., activated cells. (b) Schematic representation of experimental procedure. RNase treatment was performed on nuclear extracts before separating nuclear soluble (NS) and chromatin enriched (Chr) fractions. As control, samples from wild-type or tagged animals were processed in parallel but omitting the RNA endonucleases (mock). (c) Western blot to sub-cellular fractions following RNase treatment of nuclear extracts shown in (B).
