Extended Data Fig. 9: Loss of Ago2 catalytic competence does not result in overt changes in H3K9me3.
From: AGO2 silences mobile transposons in the nucleus of quiescent cells

(a) Western blot to AGO2 following CRE-mediated locus recombination. (b) Left, representation of the outcome of the recombination experiment. Right, relative expression levels of IAP and MERVL following conditional loss of AGO2 in resting B cells. Data are presented as mean of biological duplicates +/− SD. p values were calculated with a two-sided t-test. (c) Profile plots showing normalized read counts for H3K9me3 ChIP-seq over two example young L1 repeats following CRE-mediated recombination of Ago2flx/+ and Ago2flx/ADH in resting B cells and Ago2+/+ controls. (d) H3K9me3 ChIP signal depth over consensus repeat sequences. (e) Enrichment for H3K9me3 (calculated as log2 of the ratio between ChIP and input) for repeats of the L1MdA (top), L1MdTf, and L1MdGf (bottom) young L1 families following CRE-mediated recombination of Ago2flx/+ and Ago2flx/ADH in resting B cells and Ago2+/+ controls. Boxplots show minimum, maximum, median, first, and third quartiles from two biological replicates whose individual values are represented by a dot.