Extended Data Fig. 4: Nocodazole, but not STLC, disrupts mitotic ER morphology.
a, Schematic of living cell imaging of ER-GFP-labeled mitotic cells using three-dimensional structured illumination microscopy (3D-SIM). The length of ER elements was calculated using ImageJ software. b, Left, representative 3D-SIM images of the ER in ER-GFP-labeled MDA-MB-231 cells treated with DMSO, nocodazole, or STLC. Red boxed regions were enlarged and subjected to 3D reconstruction. Scale bars = 5 μm. Right, the length of ER elements from multiple cells in the optical SIM sections. DMSO, n = 8; Nocodazole, n = 8; STLC, n = 7. Data from b were examined over three independent experiments, and analyzed using one-way Welch’s ANOVA test (P < 0.0001), pairwise comparisons were performed using Games-Howell’s multiple comparisons test. Data are presented as mean ± s.e.m.
