Extended Data Fig. 2: Structural analysis of Rab1AThr75 phosphorylation. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Structural analysis of Rab1AThr75 phosphorylation.

From: Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

Extended Data Fig. 2

a, Structural overlay of the two molecules (chains) in the asymmetric unit of the Rab1A-T75D crystal. Switch II region is specified. Note the consistent configuration of T75D in the two chains. b, Local interactions of the switch II loop (coloured magenta) in the crystal packing of Rab1A-T75D. The two crystallographic Rab1A-T75D dimers are coloured cyan and grey, respectively. NC, non-crystallographic. c, Comparison of the nucleotide binding pockets of WT Rab1A (PDB code 2FOL) and Rab1A-T75D. Note that R72 (yellow) blocks the exit of the pocket in Rab1A-T75D. d, Structural comparison between Rab1A-T75D-•GDP and Rab8A-Q67L-pT72•GTP:RILPL2 complex (PDB code 6RIR). Note the different conformations of switch II in Rab1A and Rab8A. e, Complex structures of Rab1A•GDP•AlF3 with Shigella VirA (left, 4FMB), E. Coli EspG (middle, 4FMD), and Legionella LepB (right, 4JVS). Note the different distances between Rab1A Thr75 and these GAPs.

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