Extended Data Fig. 4: Pol II mutants alter TSS efficiency in general. | Nature Structural & Molecular Biology

Extended Data Fig. 4: Pol II mutants alter TSS efficiency in general.

From: Quantitative analysis of transcription start site selection reveals control by DNA sequence, RNA polymerase II activity and NTP levels

Extended Data Fig. 4

(A) TSS efficiency distributions of designed +1 TSSs of Pol II mutants for base subgroups at individual positions relative to +1. Identical analysis as in Extended Data Fig. 2B for WT Pol II. (B) Pol II GOF G1097D showed greater increase in efficiency than GOF allele E1103G at upstream TSSs (designed −32 and −8 TSSs), while E1103G showed stronger effects at designed +1 TSS than G1097D. (C) Pol II initiation sequence preference in Pol II mutants. Identical analysis as in Fig. 3B for WT Pol II. (D) Motif enrichment for top the 10% most efficient −8 TSSs for Pol II mutants. Identical motif enrichment analysis as in Extended Data Fig. 2D top panel for WT Pol II. Numbers (N) of variants assessed are indicated. Bars represent an approximate Bayesian 95% confidence interval.

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