Extended Data Fig. 4: Statistics of D110C clamp loader deep mutagenesis screen.
From: Autoinhibition of a clamp-loader ATPase revealed by deep mutagenesis and cryo-EM
a, Agreement between fitness measurements from three replicate experiments per assay pool (residues 2-115 assay pool n=1130, and residues 115-230 assay pool n=2076) is shown in scatter plots. Each point in the scatter plot represents the fitness measurements made from the two trials. b, The histogram shows the spread of relative fitness values for the AAA+ module of the ATPase subunit in the background of the D110C mutant clamp loader. c, Small-scale libraries of recovery mutations in the background of D110C were constructed and subjected to the phage replication assay. The fitness of this small library per substitution (x-axis) is plotted against the fitness scores of same mutations as assayed in the full-scale deep mutagenesis screen (y-axis). Data are presented as mean values +/− standard deviations (SD), n=3 biological replicates.
