Fig. 3: Supercoiling and contacts resulting from combination of pairs of transcription units. | Nature Structural & Molecular Biology

Fig. 3: Supercoiling and contacts resulting from combination of pairs of transcription units.

From: Transcription-induced domains form the elementary constraining building blocks of bacterial chromosomes

Fig. 3

a–f, Genomic characterization of chromosomal regions carrying pairs of pT7 promoters in different orientations. The orientations are as follows: a single promoter (a), two divergent promoters at 100 kb (b), 60 kb (c), two convergent promoters at 100 kb (d), two unidirectional (collinear) promoters at 100 kb (e) and 60 kb (f). From top to the bottom: Hi-C contact map (first row; bin: 1 kb), RNA-seq track (second row; in CPM), T7 RNA Pol ChIP–seq track (third row, blue curve) and short-range Hi-C contacts (third row, red curve), and GapR ChIP–seq revealing positive supercoiling (fourth row, yellow curve) and short-range Hi-C contacts (fourth row, red curve). Values on the top right corner of each panel are the Spearman correlation coefficients of the track with the short-range Hi-C contacts. All tracks are z-transformed.

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