Extended Data Fig. 4: Size-exclusion chromatography and negative staining EM micrographs of CRL3KBTBD2 mutants.
From: Dynamic molecular architecture and substrate recruitment of cullin3–RING E3 ligase CRL3KBTBD2
a, Size-exclusion chromatography (SEC) comparison of CRL3KBTBD2 mutants on H24 (Q489A, H490A, Q492A, T494A, V496A), H25 (R537A, K542A, S544A), WHB (S713A, R714A, K715A, K716A). The chromatographic separation of the standard proteins is shown as a grey line and the theoretical molecular weights are shown above. b-f, Coomassie-stained SDS-PAGE gel (Left) and negative-stain EM analysis (Right) of the CRL3KBTBD2_WT (b), CRL3KBTBD2_H24 (c), CRL3KBTBD2_H25 (d), CRL3KBTBD2_WHB (e), CRL3KBTBD2_H2425 (f), elution fractions. The purification was repeated independently more than 3 times with similar results.
