Extended Data Fig. 7: Structure analysis of CRL3KBTBD2~N8 structure.
From: Dynamic molecular architecture and substrate recruitment of cullin3–RING E3 ligase CRL3KBTBD2
a, Cryo-EM density map and models of the CUL3CTD and RBX1 in CRL3KBTBD2~N8 structure. The subdomains of CUL3CTD are colored individually. The neddylation site Lys712 is shown as green spheres. b, Neddylation assay of CRL3KBTBD2. n, the number of biological independent replicates. c-e, Structural comparison of CRL3KBTBD2~N8 with other neddylatd CRLs: CUL1~N8 (PDB: 6TTU) and CUL5 ~N8 (PDB: 3DQV). The N8 sides are shown as atoms in green. f-h, Fitting CRL3KBTBD2~N8 into CRL3KBTBD2 multimer maps indicates structural clashes (circled) between RBX1 and CUL34HB subdomain from the neighboring CUL3CTD.
