Extended Data Fig. 10: Structure of the CUL3–RBX1–CAND1 complex.
From: Dynamic molecular architecture and substrate recruitment of cullin3–RING E3 ligase CRL3KBTBD2
a, The schematic representation of the domain composition of CAND1. b, Cryo-EM density of CAND1 and ribbon model of the CUL3–RBX1–CAND1 complex. The neddylation site K712 in WHB subdomain is shown in green spheres. c, d, Superimposing KBTBD2BTB-BACK/CUL3NTD structure with CUL3–RBX1–CAND1, showing that CAND1 clashes with BTB domain. The KBTBD2BTB-BACK is shown in gray surface. e, the interface between CAND1 N-terminal arch and CUL3WHB. f, Superimposition of the CUL3–RBX1–CAND1 model (multi-colored) with CUL1–RBX1–CAND1 (PDB: 1U6G, gray) and CUL4B–RBX1–CAND1 (PDB: 4AOC, blue).
