Extended Data Fig. 1: Size exclusion chromatography and negative stain micrographs of KBTBD2, p85α, CRL3KBTBD2, CRL3KBTBD2–p85α and CRL3KBTBD2~N8.
From: Dynamic molecular architecture and substrate recruitment of cullin3–RING E3 ligase CRL3KBTBD2
a–e, Size exclusion chromatography and negative stain micrographs of KBTBD2 (a), p85α (b), CRL3KBTBD2 (c), CRL3KBTBD2–p85α (d) and CRL3KBTBD2~N8 (e). Left: SEC profiles, the chromatographic separation of the standard proteins is shown as a grey line and the theoretical molecular weights are shown above. Middle: Coomassie−stained SDS-PAGE analysis of the peak fraction of SEC. Right: representative micrographs. The purification was repeated independently more than 3 times with similar results.
