Extended Data Fig. 2: Size exclusion chromatography and negative stain micrographs of CSN in complex with neddylated and non-neddylated CRL3KBTBD2.
From: Dynamic molecular architecture and substrate recruitment of cullin3–RING E3 ligase CRL3KBTBD2
a, CRL3KBTBD2. b, CSN. c, CSNH138A. d, CRL3KBTBD2–CSN. e, CRL3KBTBD2~N8Δ–CSN. f, CRL3KBTBD2~N8–CSNH138A. Left: SEC profiles, the chromatographic separation of the standard proteins is shown as a grey line and the theoretical molecular weights are shown above. Middle: Coomassie−stained SDS-PAGE analysis of the peak fraction of SEC. Right: representative micrographs. The purification was repeated independently more than 3 times with similar results.
