Extended Data Fig. 3: Structure of the CRL3KBTBD2 monomer.
From: Dynamic molecular architecture and substrate recruitment of cullin3–RING E3 ligase CRL3KBTBD2
a, Ribbon representation of CRL3KBTBD2 monomer with subdomains colored differently. b, The cryo-EM map and the model of the CUL3CTD and RBX1, showing that the N-terminal β-strand of RBX1 (RBX1NTD) inserts into the CUL3CTD, forming a stable β-sheet. The subdomains of the CUL3CTD are colored individually. Three zinc ions in RBX1 are shown as green spheres. c, Analysis of interface between KBTBD2 and CUL3. The residues located at the contact surface are shown as sticks. d, Ribbon representation of the KBTBD2Kelch domain in side and top views. The Kelch domain contains six kelch-repeats and each consists of four β-strands, packing into a helix cluster structure. e, Superposition of five Kelch domains: KLHL12 (PDB: 2VPJ), KLHL2 (PDB: 2XN4), KBTBD10 (PDB: 2WOZ), KEAP1 (PDB: 2FLU), KBTBD2 (this study). f-g, Sequence and structural alignment of BTB domain of KBTBD2 with another four BTBs.
