Extended Data Fig. 3: Cryo-EM analysis of NTCP/myr-preS1 complex.
From: Structural basis of hepatitis B virus receptor binding
a, Representative size-exclusion chromatography of the human NTCP/myr-preS1 complex. Absorbance profiles (top) and SDS-PAGE analysis after staining with Coomassie Brilliant Blue (CBB, bottom) are shown. The pooled fractions are indicated. Absorbances at 280 nm and 260 nm are shown as solid and dashed lines, respectively. Purifications were performed twice. b, c, Data processing workflow of cryo-EM analysis of human NTCP/myr-preS1 in complex with YN9048Fab (b) and YN9016Fab (c). Representative motion-corrected micrographs (out of 11,785 (b) and 5,711 (c) total micrographs), 2D class averages, gold-standard FSC curves of the final 3D reconstruction (resolution cut-off at FSC = 0.143), and the final 3D map (colored according to the local resolution) are shown. The 2D class averages were calculated using refined particles that were used for the final reconstruction.
