Extended Data Fig. 1: Biochemical preparation of GPR161-miniGs complex. | Nature Structural & Molecular Biology

Extended Data Fig. 1: Biochemical preparation of GPR161-miniGs complex.

From: GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway

Extended Data Fig. 1

a) Cartoon depiction of GPR161 stabilization, solubilization, and purification. b) Size-exclusion chromatogram (left) and SDS-PAGE gel (right) of purified GPR161-Gs complex with Nb35. Purification and SDS-PAGE gel were done once and not repeated. Chromatogram and SDS-PAGE gel are shown for preparation used for cryo-EM analysis.

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