Extended Data Fig. 2: Cryogenic electron microscopy processing of GPR161. | Nature Structural & Molecular Biology

Extended Data Fig. 2: Cryogenic electron microscopy processing of GPR161.

From: GPR161 structure uncovers the redundant role of sterol-regulated ciliary cAMP signaling in the Hedgehog pathway

Extended Data Fig. 2

a) A representative motion-corrected cryogenic electron microscopy (cryo-EM) micrograph obtained from a Titan Krios microscope (n = 8,294). b) A subset of highly populated, reference-free 2D-class averages. c) Schematic showing the cryo-EM data processing workflow. Initial processing was performed using UCSF MotionCor2 and cryoSPARC. Particles were transferred using the pyem script package to RELION for alignment-free 3D classification. Finally, particles were processed in cisTEM using the manual refinement job type with a 7TM mask followed by a full particle mask. Dashed boxes indicated selected classes. d) Gold-standard Fourier Shell Correlation (GSFSC) curve for final refined and sharpened map computed in cryoSPARC. e) Euler angle distribution of final refined map computed in cryoSPARC.

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