Extended Data Fig. 3: Cryo-EM reconstruction of PI3Kγ·ADP–GβγHD–GβγCTD in state1.
From: Molecular basis for Gβγ-mediated activation of phosphoinositide 3-kinase γ
(A) Cryo-EM map of state 1 with domain colored and indicated in (M). (B) Close−up of the kinase domain in state 1 with no observable density for α36. (C) Kinase domain in the structure of PI3Kγ·ADP, with α36 indicated. (D) Gβγ residues involved in the p110γ-HD interaction. (E-F) Residues in the p110γ-HD–Gβγ interface. Residues are shown as sticks and colored according to atom type. (G-I) Cryo-EM map and model fitting for the p101-CTD–GβγCTD interaction. (J) Gβγ residues involved in the p110γ-CTD interaction. (K-L) Residues in the p101-CTD–GβγCTD interface. (M) Color key for domains in p110γ, p101, and Gβγ.
