Extended Data Fig. 9: Colocalization of SID-1 and dsRNA in C. elegans.
From: Structural insights into double-stranded RNA recognition and transport by SID-1
a, Colocalization of Cy5-labeled dsRNA and YFP::SID-1 in the body-wall muscle cells. The transgenic sid-1 (qt9) worms expressing YFP::SID-1 in the body-wall muscles and SID-1 in the pharynx muscles were imaged after the injection of Cy5-labeled dsRNA into the pharynx of the worms. b, Cy5-labeled dsRNA was not detected in the body-wall muscle cells of sid-1 (qt9) worms only expressing YFP::SID-1 in the body-wall muscle. c and d, No colocalization of SID-1 and cy5 (c) or cy5-ssRNA (d) was observed. Scale bars, 100 μm (upper panels), 10 μm (lower panels). e, Fraction of colocalization of cy5-dsRNA and YFP::SID-1 in observed sid-1 (qt9) worms. The number of independent repetitions in a-d is marked in the bar chart in panel e.
